Ho bioprocessing, ho boloka taolo e nepahetseng ea maemo a tikoloho ho bohlokoa.Maemo ana a bohlokoa ka ho fetisisa ke pH, e susumetsang kholo le tlhahiso ea likokoana-hloko kapa lisele tse sebelisoang lits'ebetsong tse fapaneng tsa biotechnology.Ho fihlela taolo ena e nepahetseng, basebetsi ba bioreactor ba itšetleha ka lisebelisoa tse tsoetseng pele le li-sensor - ea bohlokoa ka ho fetesisa kebioreactor pH sensor.
Bioreactor pH Sensor: Melawana ya Motheo ya Tekanyo ya pH
1. Bioreactor pH Sensor: Tlhaloso ea pH
pH, kapa "mokhoa oa hydrogen," ke tekanyo ea asiti kapa alkalinity ea tharollo.E lekanya bongata ba li-ion tsa hydrogen (H +) ka tharollo e fanoeng 'me e hlahisoa ka tekanyo ea logarithmic ho tloha ho 0 ho ea ho 14, le 7 e emelang ho se nke lehlakore, litekanyetso tse ka tlase ho 7 tse bontšang acidity, le litekanyetso tse ka holimo ho 7 tse bontšang alkalinity.Ho bioprocessing, ho boloka boemo bo itseng ba pH ho bohlokoa bakeng sa kholo e nepahetseng le tlhahiso ea likokoana-hloko kapa lisele.
2. Bioreactor pH Sensor: pH Scale
Ho utloisisa sekala sa pH ho bohlokoa ho utloisisa bohlokoa ba tlhahlobo ea pH.Sebopeho sa logarithmic sa sekala se bolela hore phetoho ea yuniti e le 'ngoe e emela phapang e imenneng leshome ka har'a mahloriso a hydrogen ion.Kutloisiso ena e etsa hore taolo e nepahetseng ea pH e be ea bohlokoa ho li-bioreactors, moo liphapang tse nyane li ka amang bioprocess haholo.
3. Bioreactor pH Sensor: Bohlokoa ba pH Monitoring ho Bioprocessing
Bioprocessing e akaretsa lits'ebetso tse fapaneng, ho kenyelletsa ho belisoa, tlhahiso ea biopharmaceutical, le kalafo ea metsi a litšila.Ho e 'ngoe le e 'ngoe ea lits'ebetso tsena, ho boloka mofuta o itseng oa pH ho bohlokoa bakeng sa ho laola karabelo ea enzymatic, kholo ea likokoana-hloko, le boleng ba sehlahisoa.Tlhokomelo ea pH e netefatsa hore tikoloho ea bioreactor e lula e le ka har'a maemo a lakatsehang, e ntlafatsa tlhahiso le chai ea sehlahisoa.
4. Sensor ea pH ea Bioreactor: Lintlha tse Amang pH ho Li-Bioreactors
Lintlha tse 'maloa li ka ama maemo a pH ka har'a li-bioreactors.Tsena li kenyelletsa tlatsetso ea lintho tse asiti kapa tsa alkaline, lihlahisoa tsa metabolism ea likokoana-hloko, le liphetoho tsa mocheso.Ho beha leihlo le ho laola mefuta ena ea lintho ka nako ea 'nete ho khoneha ke li-sensor tsa pH, tse bapalang karolo ea bohlokoa taolong ea bioprocess.
Bioreactor pH Sensor: Mefuta ea li-PH Sensors
1. Bioreactor pH Sensor: Glass Electrode pH Sensors
Li-sensor tsa pH tsa electrode tsa khalase ke e 'ngoe ea mefuta e atileng haholo e sebelisoang ts'ebetsong ea bioprocessing.Li na le lera la khalase le arabelang liphetohong tsa hydrogen ion concentration.Li-sensor tsena li tsebahala ka ho nepahala le ho ts'epahala ha tsona, e leng se etsang hore e be khetho e ratoang bakeng sa lits'ebetso tsa bohlokoa tsa bioreactor.
2. Bioreactor pH Sensor: ISFET (Ion-Selective Field-Effect Transistor) pH Sensors
Li-sensor tsa ISFET pH ke lisebelisoa tsa boemo bo tiileng tse bonang liphetoho tsa pH ka ho metha voltage ho silicon chip.Ba fana ka melemo e kang ho tšoarella le ho tšoaneleha bakeng sa lits'ebetso tsa ts'ebeliso e le 'ngoe, ho ba etsa khetho e fapaneng ho bioprocessing.
3. Bioreactor pH Sensor: Reference Electrodes
Li-electrode tsa litšupiso ke karolo ea bohlokoa ea li-sensors tsa pH.Li fana ka monyetla o tsitsitseng oa litšupiso oo electrode ea khalase e lekanyang pH.Khetho ea electrode ea referense e ka ama ts'ebetso ea sensor, 'me ho khetha motsoako o nepahetseng ho bohlokoa bakeng sa tekanyo e nepahetseng ea pH.
4. Sensor ea pH ea Bioreactor: Papiso ea Mefuta ea Sensor
Ho khetha sensor e nepahetseng ea pH bakeng sa ts'ebeliso ea bioprocessing ho ipapisitse le lintlha tse joalo ka ho nepahala, ho tšoarella, le ho lumellana le litlhoko tse ikhethileng tsa ts'ebetso.Papiso ea mefuta e fapaneng ea li-sensor e tla thusa litsebi tsa bioprocess ho etsa liqeto tse nang le tsebo ha li khetha lisebelisoa tsa ho lekola pH.
Bioreactor pH Sensor: Bioreactor pH Sensor Design
1. Bioreactor pH Sensor: Sensor Housing
Ntlo ea sensor ke khetla e ka ntle e sireletsang likarolo tse ka hare ho tikoloho e thata ka har'a bioreactor.Ha u khetha thepa bakeng sa ntlo, ke habohlokoa ho nahana ka lintlha tse kang ho lumellana ha lik'hemik'hale, ho tšoarella ha nako le ho hloekisa habonolo.Tšepe e sa hloekang ke thepa e sebelisoang hangata ka lebaka la ho hanyetsa ho bola le ho tiea.Sebopeho le boholo ba ntlo li lokela ho raloa ho lumellana le litlhoko tse khethehileng tsa bioreactor ha ho ntse ho etsoa bonnete ba ho kenya le tlhokomelo habonolo.
2. Bioreactor pH Sensor: Sensing Element
Pelo ea sensor ea pH ke karolo ea eona ea kutlo.Li-sensor tsa pH tsa bioreactorhangata ba sebelisa electrode ea khalase kapa Ion-Sensitive Field Effect Transistor (ISFET) e le ntho ea kutlo.Li-electrode tsa khalase li tsejoa ka ho nepahala le ho tšepahala ha tsona, ha ISFETs e fana ka melemo ho latela miniaturization le ho tiea.Khetho pakeng tsa tsena tse peli e itšetlehile haholo ka litlhoko tsa kopo.Ho khetha tharollo e nepahetseng ea electrolyte ka har'a ntho ea kutlo ho bohlokoa bakeng sa ho boloka ts'ebetso ea li-electrode ha nako e ntse e ea.
3. Bioreactor pH Sensor: Reference Electrode
Electrode ea referense e bohlokoa bakeng sa tekanyo ea pH kaha e fana ka sebaka se tsitsitseng sa litšupiso.Ho na le mefuta e fapaneng ea li-electrode tsa litšupiso, ho kenyeletsoa li-electrode tsa Ag/AgCl le Calomel.Maikutlo a tlhokomelo a kenyelletsa ho boloka motsoako oa electrode o hloekile le ho netefatsa hore tharollo ea litšupiso e lula e tsitsitse.Ho hlahloba khafetsa le ho tlatsa tharollo ea litšupiso hoa hlokahala ho boloka ho nepahala.
4. Sensor ea pH ea Bioreactor: Moralo oa Junction
Moralo o kopaneng oa sensor ea pH o bohlokoa bakeng sa ho boloka phallo ea li-ion lipakeng tsa tharollo ea ts'ebetso le electrode ea referense.Moralo ona o lokela ho thibela ho koala le ho fokotsa ho thella ho baleng.Khetho ea lisebelisoa tsa matekoane le tlhophiso ea eona e bapala karolo ea bohlokoa ts'ebetsong ea kakaretso ea sensor.
6. Sensor ea pH ea Bioreactor: Mekhoa ea ho lekanya
Calibration ke mohato oa bohlokoa ho netefatsa litekanyo tse nepahetseng tsa pH.Li-sensor tsa pH li lokela ho lekanyetsoa khafetsa ho sebelisoa litharollo tse tloaelehileng tsa buffer tse nang le boleng bo tsebahalang ba pH.Mekhoa ea ho lekanya e lokela ho lateloa ka hloko, 'me lirekoto tsa ho lekanya li lokela ho bolokoa molemong oa ho latelloa le ho laola boleng.
Bioreactor pH Sensor: Ho kenya le ho kopanya
1. Bioreactor pH Sensor: Ho behoa ka hare ho Bioreactor
Ho beoa ka nepo ha li-sensor tsa pH ka har'a bioreactor ho bohlokoa ho fumana litekanyo tse emelang.Li-sensor li lokela ho behoa sebakeng se nepahetseng ho lekola phapang ea pH ho pholletsa le sekepe.Ho kenngoa ho boetse ho lokela ho nahana ka lintlha tse kang ho shebana le sensor le sebaka sa ho tloha ho agitator.
2. Sensor ea pH ea Bioreactor: Khokahano le Litsamaiso tsa Taolo
Li-sensor tsa pH tsa bioreactor li tlameha ho kenngoa tsamaisong ea taolo ea bioreactor.Sena se kenyelletsa ho hokahanya sensor ho transmitter kapa controller e ka tolokang lipalo tsa pH le ho etsa liphetoho tse hlokahalang ho boloka boemo ba pH bo lakatsehang.
3. Sensor ea pH ea Bioreactor: Lintlha tsa Cable le Connector
Ho khetha likhoele tse nepahetseng le lihokelo ho bohlokoa bakeng sa phetiso ea data e tšepahalang le bophelo bo bolelele.Lithapo li lokela ho etsoa ho mamella maemo a thata ka har'a bioreactor, 'me likhokahano li tlameha ho hana ho bola ho boloka khokahanyo e tsitsitseng ea motlakase.
Bioreactor pH Sensor: Calibration le Tlhokomelo
1. Sensor ea pH ea Bioreactor: Mekhoa ea ho lekanya
Ho lekanya khafetsa ho bohlokoa ho netefatsa litekanyo tse nepahetseng tsa pH.Khafetsa ea ho lekanya e ipapisitse le lintlha tse kang botsitso ba sensor le bohlokoa ba taolo ea pH ts'ebetsong.Ho khothalletsoa ho latela tataiso ea moetsi bakeng sa mekhoa ea ho lekanya.
2. Bioreactor pH Sensor: Frequency of Calibration
Khafetsa ea calibration e lokela ho khethoa ho latela ts'ebeliso e khethehileng le botsitso ba sensor.Li-sensor tse ling li ka hloka ho lekoa khafetsa, ha tse ling li ka boloka ho nepahala ha nako e telele.
3. Sensor ea pH ea Bioreactor: Ho Hloekisa le Tlhokomelo
Ho hloekisa le tlhokomelo e nepahetseng ke tsa bohlokoa bakeng sa bophelo bo bolelele ba sensor le ho nepahala.Mekhoa ea ho hloekisa e lokela ho etsoa khafetsa ho tlosa biofilm leha e le efe kapa li-deposit tse ka bokellang holim'a sensor.Tlhokomelo e boetse e kenyelletsa ho hlahloba electrode ea litšupiso le mateano bakeng sa matšoao a ho senyeha kapa ho silafala.
4. Sensor ea pH ea Bioreactor: Ho rarolla mathata a tloaelehileng
Leha ho na le moralo le tlhokomelo e nepahetseng, li-sensor tsa pH li ka kopana le mathata a kang ho hoholeha, lerata la lets'oao, kapa ho silafatsoa ha electrode.Mekhoa ea ho rarolla mathata e lokela ho ba teng ho khetholla le ho rarolla mathata ana hang-hang ho fokotsa tšitiso ea ts'ebetso.
Qetello
Thebioreactor pH sensorke sesebelisoa sa bohlokoahali sa ts'ebetso ea bioloji, se lumellang taolo e nepahetseng ea maemo a pH ho ntlafatsa kholo ea likokoana-hloko le chai ea lihlahisoa.Ho utloisisa melao-motheo ea ho lekanya pH le mefuta e fapaneng ea li-sensor tsa pH tse fumanehang ho matlafatsa basebelisi ba bioprocess ho etsa liqeto tse nepahetseng mabapi le ho khetha lisebelisoa tse loketseng lits'ebetso tsa bona.Ka li-sensor tse tšepahalang tsa pH tse tsoang ho bafani ba joalo ka Shanghai BOQU Instrument Co., Ltd., litsebi tsa bioprocessing li ka tsoela pele ho ntšetsa pele lefapha la theknoloji ea baeloji le ho hlahisa lihlahisoa tsa boleng bo holimo ka nepo.
Nako ea poso: Sep-13-2023
